Pituitary adenylate cyclase activating peptide (PACAP), originally isolated from ovine hypothalamus, belongs to the VIP-family of peptides.
PACAP-related peptide (PRP) is a 29aa peptide, which is produced together with PACAP-27 and PACAP-38 when Prepro PACAP is being processed.
PRP is abundant in the brain, but can be also found in the respiratory and gastrointestinal tracts.
Absorption with 10-100ug immunogen per ml diluted antiserum abolishes the staining, while PACAP-27, PACAP-38, VIP, PHI, CRF, oxytocin and vasopressin do not.
Applications:Suitable for use in Immunofluorescence microscopy (frozen sections).
Recommended Dilution:Immunofluorescence microscopy: 1:400-1:800 with overnight incubation at 2-8°C.
Positive Control:Stefanini-fixed frozen sections of rat duodenum or hypothalamus.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder.
Specificity：Recognizes PACAP-Related Peptide. Species Crossreactivity: Human and rat.
Isotype：IgG
Calc Applications Abbrev：IF
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic rat PRP (Pensinsula), conjugated to BSA
