Ornithine decarboxylase is the rate-limiting enzyme in polyamine biosynthesis converting ornithine into putrescine.
In normal tissue ornithine decarboxylase activity is low but increases in proliferating tissue.
Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunofluorescence: 1:500-1:800 with overnight incubation at 4°C
Immunohistochemistry: Frozen tissue sections and on paraffin embedded material when using the microwave retrieval method
Optimal dilutions to be determined by the researcher.
Positive Control:Stefanini-fixed frozen sections of renal cortex from testosterone-treated mice.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ul
Host:guinea pig
Source Antibody:human
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder. Reconstitute with 50-100ul ddH2O. Make further dilutions with PBS, 0.5% BSA, 0.01% sodium azide.
Specificity:Recognizes human Ornithine Decarboxylase. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes staining. Species Crossreactivity: mouse and rat.