Located in the cell membrane of thyroid cells, NIS can allow sodium and iodine flow across the membrane.
Suitable for studying the sensitivity of thyroid tumors to iodine treatment..
Applications:Suitable for ELISA, Immunohistochemistry and Immunofluorescence Microscopy.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Frozen sections): 1:1200-1:2000 using FITC conjugates as secondary antibodies; Incubation Time Overnight at 4°C
Optimal dilution to be determined by researcher.
Positive Control:Frozen sections of rat thyroid.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute in 50-100ul of sterile ddH2O. Dilute further with 0.1M PBS, 1% BSA, 0.09% sodium azide.
Specificity：Recognzies rat NIS. Species Crossreactivity: porcine and human.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu Po Rt
Immunogen：Synthetic peptide corresponding to the C-terminus of rat NIS.
