Nephrin was first described as a kidney podocyte marker protein with a MW of 135kD calculated from amino acid sequence data and an apparent Mr 185kD after SDS-PAGE.
Nephrin is a transmembrane cell adhesion molecule located at the slit diaphragm.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry (Paraffin and Frozen): 1:50 Microwave antigen retreval required on paraffin sectionsWestern Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：guinea pig
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.09% sodium azide.
Specificity：Recognizes mouse nephrin. Positively stains podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas. Reacts with PCL (podocyte cell line) and M-1 (cortical collecting duct cells). Species Crossreactivity: human
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the intracellular domain, aa1243-1256, EPGSLPFELRGHLV, of mouse Nephrin.
