84-1063-15　Laminin 250ul　L1225-09

特徴

• Laminin is a glycoprotein (Mr 850-1,000kD, consisting of 3 glycosylated polypeptide chains with molecular weights of 440 and 225 (2x) kD) produced by various human epithelial and mesenchymal cells, and forms an extracellular matrix of thin filaments.
• In normal tissues, laminin is invariably present in all basal laminas surrounding muscle, nerve, fat and decidua cells and separates epithelial and endothelial cells from abutting connective tissues.
• Laminin has also been identified within the cytoplasm of breast epithelia, stromal cells of the endometrium, and within endothelial, bile duct epithelial and mesenchymal cells of the liver.
• Laminin has been found to be involved in cellular activities such as adhesion, spreading, differentiation, polarization, proliferation, locomotion, tissue invasion and chemotactic responses.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1:50-1:100Immunohistochemistry: 1:50-1:00 Frozen and paraffin tissues (proteolytic treatment required; enzyme: pepsin)Optimal dilutions to be determined by the researcher.
• Positive Control:Skin, kidney
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.