Insulin Receptor Substrate (IRS-1) is one of the major substrates of Insulin Receptor Kinase.
IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain-containing proteins (Grb2, p85, SHP-2, Fyn and others) and which mediate the metabolic and growth promoting functions of insulin.
IRS-1 also contains over 30 potential Ser/Thr phosphorylation sites for kinases, such as PKA, PKC and MAPK.
The phosphorylation of Ser 612 and Ser636/639 are mediated by PKA and mTOR pathways, respectively, resulting in an inhibition of insulin signaling in the cell and suggesting a potential mechanism for insulin resistance in some models of obesity.
Once phosphorylated by insulin receptor, IRS-1 recruits the downstream components that mediate the insulin signaling.
IRS-1 protein contains a conserved pleckstrin homology (PH) domain located at its amino terminus, adjacent to a phospho-tyrosine binding (PTB) domain.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：guinea pig
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.09% sodium azide, 0.5% BSA.
Specificity：Recognizes human keratin K71 (57.3kD polypeptide; calculated from sequence data). The antiserum stains specifically the Henle, Huxley and cuticle layer of the inner root sheath (irs) of the hair follicle. Although there is a considerable sequence homology of keratin K71 to keratins K5 and K6, no crossreactivity was observed with Western Blot and immunofluorescence microscopy. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide corresponding to residues aaC-GGEGRSRGSANDYKDT (formerly also designated keratin K6irs1) from human keratin K71.
