Insulin is produced by the B-cells of the pancreatic islets.
Antigen localization: cytoplasm, extracellular.
Applications:Suitable for use in ELISA and Immunohistochemistry (frozen and paraffin sections, Proteolytic treatment not required; optimal results have been obtained by using the autoclave or microwave retrieval method (with citrate buffer))
Recommended Dilution:Immunohistochemistry: 1:50 -1:100; Based on the indirect method, with a normal ABC method the dilution can be up to 5X higher when using the microwave retrieval method, but the optimal dilution should be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:250ul
Host:rabbit
Source Antibody:porcine
Grade:Serum
Purity:Serum
Form:Supplied as a liquid.
Specificity:Can be used for the diagnosis of pancreatic B-cell tumors. Recommended for Pancreas. Species Crossreactivity: Human, porcine, rat, ferret.