Glicentin contains the glucagon sequence and is produced in a prominent population of endocrine cells in the distal intestine as well as in pancreatic glucagon cells and in the nerves in the brain.
Serum levels of glicentin are elevated after food uptake and in certain clinical conditions, e.g.
after resections of the intestine.
The functional role of glicentin is largely unknown.
Glicentin occurs in endocrine tumors arising in the distal intestine (rectal carcinoids) and in pancreatic islet cell tumors.
Applications:Suitable for use in Immunohistochemistry and Immunofluorescence.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry (frozen and paraffin): 1:2000-1:3000 (PAP)
Immunofluorescence: 1:1000-1:1500 with overnight incubation at 2-8°C
Optimal dilutions to be determined by the researcher.
Positive Control:Formalin-fixed paraffin sections of porcine pancreas
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：porcine
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 50-100ul sterile ddH2O. Dilute further with PBS, 1% BSA, 0.09% sodium azide.
Specificity：Recognizes porcine Glicentin/Glucagon. Absorption with 10-100ug glucagon and glicentin per ml diluted antiserum abolishes the staining, while secretin, GIP and VIP do not. Species Crossreactivity: human, rat, hamster.
Isotype：IgG
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Hm Hu Po Rt
Immunogen：Porcine pancreatic glucagon, conjugated to BSA.
