Enkephalins are small peptides derived from large precursers (pro-enkephalin A and B) containing multiple enkephalin copies.
They are the most abundant opioid peptides in the body and are widely distributed in the brain and the peripheral nervous system and occur also in the adrenal medulla.
Several types of neuroendocrine tumors, incl.
pheochromocytomas, neuroblastomas and bronchial and gastrointestinal endocrine tumors, produce enkephalin.
Applications:Suitable for use in Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Frozen/paraffin): 1:400-1:800 using FITC and incubated overnight at 4°C
Optimal dilutions to be determined by the researcher.
Positive Control:Frozen sections of feline and porcine small intestine
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 50-100ul sterile ddH2O. Dilute further in PBS, 1% BSA, 0.09% sodium azide
Specificity：Recognizes Enkephalin. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes the staining, while beta endorphin does not. Does recognize leu-enkephalin. Species Crossreactivity: porcine, feline and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Fe Po Rt
Immunogen：Synthetic met-enkephalin conjugated to BSA.
