Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (frozen and paraffin sections): 1:300-1:600 (FITC, immunofluorescence microscopy) with overnight incubation at 2-8°C
Optimal dilution determined by the researcher.
Positive Control:Formalin-fixed paraffin sections of pig pituitary.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile buffer or ddH2O.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder (serum). Reconstitute with 100ul distilled water to prepare the stock solution. Further dilutions can be made with 0.1M PBS containing 1% BSA and 0.09% sodium azide.
Specificity:Recongizes human beta-Endorphin. Absorption with 10-100ug beta-endorphin per ml diluted antiserum abolishes the staining, while beta-lipotropin, met-enkephalin and leu-enkephalin do not. Species Crossreactivity: porcine and rat.
Isotype:IgG
Calc Applications Abbrev:IF IHC
Calc Crossreactivity:Hu Po Rt
Immunogen:Synthetic peptide corresponding to human beta-Endorphin