Drebrin has originally been described only in neuronal cells, but has been recently found also in the actin-rich lamellipodia and filopodia of motile cells.
It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells.
Positive staining was found in many tissues, including diverse epithelia and carcinoma (e.g.
in epidermis basal cells are positive, whereas overlying cells are negative; also positive: regenerating epithelium during wound healing; basal cell carcinoma; skin melanoma), specific types of endothelia and smooth muscle; especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis.
Also positive: epithelia of hair follicles and eccrine sweat glands.
Consistently negative were, however, hepatocytes and cross-striated muscle.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence microscopy and Immunohistochemistry.
Incubate 1 hr at RT (extended with paraffin sections).
Microwave method highly recommended
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：guinea pig
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.09% sodium azide.
Specificity：Recognizes human drebrin, a widespread actin-associating protein of 70kD (SDS-PAGE mobility shows Mr 120,000). Species Crossreactivity: bovine, rat and mouse. Reactivity on Cultured Cell Lines: PLC, U333, HUVEC, HaCat, HeLa, MCF-7, SV-80; B1; B16 (mouse melanoma cells)
Isotype：IgG
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa254-272 from human Drebin, conjugated to KLH.
