84-1062-99　Drebrin 100ul　D9452

特徴

• Drebrin has originally been described only in neuronal cells, but has been recently found also in the actin-rich lamellipodia and filopodia of motile cells.
• It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells.
• Positive staining was found in many tissues, including diverse epithelia and carcinoma (e.g.
• in epidermis basal cells are positive, whereas overlying cells are negative; also positive: regenerating epithelium during wound healing; basal cell carcinoma; skin melanoma), specific types of endothelia and smooth muscle; especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis.
• Also positive: epithelia of hair follicles and eccrine sweat glands.
• Consistently negative were, however, hepatocytes and cross-striated muscle.
• Reactivities on Cultured PLC, U333, HUVEC, HaCat, HeLa, MCF-7, SV-80.
• Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence microscopy and Immunohistochemistry
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry(frozen paraformaldehyde-fixed and paraffin embedded tissue): 1:100 1 hour at RT, longer for paraffin.
• Requires microwave antigen retrieval.
• Optimal dilution to be determined by researcher.
• Positive Controls:PLC, U333, HUVEC, HaCat, HeLa, MCF-7, SV-80, B1; B16 (mouse melanoma cells).
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.