Western Blot: 1:2000 (ECL)Immunofluorescence Microscopy: 1:100-200 using acetone fixation) and 1:100 using 4% paraformaldehyde fixation
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:guinea pig
Source Antibody:human
Grade:Serum
Purity:Serum
Form:Supplied as a liquid, 0.5% BSA, 0.09% sodium azide.
Specificity:Recognizes human Cytokeratin K6hf. Stains specifically the companion layer (innermost layer of outer root sheath) of the human hair follicle. No crossreaction with cytokeratins 5 and 6, although there is considerable sequence homology. Species Crossreactivity: mouse.
Isotype:IgG
Calc Applications Abbrev:IF IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:Synthetic peptide corresponding to human type II cytokeratin, C-FTTSGGHSLGAGLGGSGFSATSNRGL conjugated to KLH.