Cytokeratin filaments consist of a complex family of approximately 20 different cytokeratin polypeptides with relative molecular weights of 40kD to 68kD.
These intracellular intermediate filaments of 10nm in diameter provide structural integrity to epithelial cells.
All the filament subunits share a general common fibrillar organization, consisting of a central alpha-helical rod that is flanked by N- and C-terminal domains at each end.
Generally, low molecular weight keratins are expressed in the early stages of cell development, whereas high molecular weight keratins are expressed in maturing cells.
This antibody may be useful in determining if a neoplasm originated from an epithelial or non-epithelial source, and is especially useful for the distinction of metastatic malignant tumors of epithelial origin.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Frozen and Paraffin): 1:50.
Incubate 1 hr at RT.
Proteolytic treatment with trypsin is recommended with paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.01% sodium azide.
Specificity：Recognizes all human stratified squamous epithelia and epidermal appendages such as hair follicles and sebaceous glands. Also positive on epithelia of the urinary tract, stomach, intestine, uterine endometrium and prostate. Identifies mesotheliomas, squamous cell carcinomas, adenocarcinomas, transitional cell carcinomas and anaplastic carcinomas. Species Crossreactivity: mouse and most mammals
