CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells).
CK9 has not been found in normal, i.e.
non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts.
No labelling has been found in epithelial cells of other stratified epithelia such as oe-sophagus or complex epithelia (e.g.
urothelium) or in ductal or simple epithelia.
Negative tissues include: muscle, liver and duodenum.
(see references listed below).
Applications:Suitable for use in Western Blot and Immunohistochemistry (frozen and paraffin-embedded cells and tissue).
Recommended Dilution:Immunohistochemistry: 1:100-1:200; 1:50 for paraffin-embedded tissue; Incubation Time 1 hr at RT; extended with paraffin
Western Blot: 1:5000-1:10,000 (ECL)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：guinea pig
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.5% BSA and 0.09% sodum azide.
Specificity：Recognizes Acidic human cytokeratin 9 (MW 62,129), expressed in the palmoplantar epidermis. The antiserum represents an excellent marker to study palmoplantar epidermal distribution and differentiation, specifically reactive in the middle/upper suprabasal layers (stratum spinosum/granulosum) of the epidermis of palm and sole. Species Crossreactivity: Human, bovine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa450-477 from human cytokeratin 9, coupled to KLH.
