Cytokeratin 17 is an excellent marker to distinguish myoepithelial cells from luminal epithelium of various glands (mammary, sweat, salivary, bronchial, tracheal, laryngeal, esophageal) and benign from malignant forms of (mammary gland tumors).
In the epidermis supratbasal stainging is found only in cornifying regions and in the outer root sheath of hair follicles.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:5000-1:10,000 (ECL)Immunohistochemistry (Frozen): 1:100-1:200; Incubate for 1 hour at RT
Immunohistochemistry (Paraffin embedded): 1:50; Incubate for > 1 hour at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:guinea pig
Source Antibody:human
Grade:Serum
Purity:Serum
Form:Supplied as a liquid, 0.5% BSA, 0.09% sodium azide.
Specificity:Recognizes acidic human Cytokeratin 17 (Mr 46kD).
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to human cytokeratin 17.