As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antiserum is useful to distinguish between non-invasive and invasive lesions.
Additionally, it can be used for the differentiation of bullous lesions in dermatopathology.
In immunohistochemistry no crossreactivity with other collagens at optimal dilutions.
In immunoblotting, a slight crossreactivity with collagen type V is observed.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Optimal dilutions to be determined by the researcher.
Recommended Positive Control:Skin and kidney
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum.
Form：Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide.
Specificity：Recognizes human collagen type IV of basement membranes and shows a homogeneous staining pattern in all tissues. In Western Blot a slight crossreactivity is observed with collagen type V.Species Crossreactivity: rat, porcine, canine, feline, fish and mouse.
