Preincubate fixed sections with 0.1 Triton X-100 (in PBS) for 1-5 minutes prior to first incubation step.
Incubation time: 1 hour at RT
Western Blot (ECL): 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：guinea pig
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.09% sodium azide.
Specificity：8/11/20 -Changed Immunogen: Synthetic peptide corresponding to GRQ IST GPS AIG GSI TVV APD C of human basic hair (trichocytic) keratin K85 (hHb5), conjugated to KLH.-Changed Specificity: Recognizes human hair keratin hHb5 expressed starting from the hair upper matrix, cortex and hair cuticle. Species Crossreactivity: mouse-FARecognizes human Basic Hair Keratin, K85 expressed starting from the hair upper matrix, cortex and hair cuticle. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to GRQISTGPSAIGGSITVVAPDC of human Basic Hair Keratin, K85 conjugated to KLH.
