Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Frozen): 1:200; Specifically stains human hair keratin KB3 expressed starting from the middle cortex.
For enhancement of cortex staining perincubate fixed sections with PBS, 0.1 Triton X-100 for 1-5 minutes prior to first incubation.
Incubate 1 hour at RT
Western Blot (ECL): 1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：guinea pig
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.5% BSA, 0.09% sodium azide.
Specificity：Recognizes human Basic Hair Keratin, K83 expressed starting from the middle cortex. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to CGFNSIGCGFRPGNF of human basic hair (trichocytic) keratin K83 conjugated to KLH. (Formally designated keratin hHb3).
