Amylin or islet amyloid polypeptide (IAPP) is produced in the pancreas beta cells and coreleased with insulin.
The amino acid sequence shows great homology with CGRP.
Amylin has been shown to reverse insulin inhibition of hepatic gluconeogenesis and to inhibit muscle uptake of glucose.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Frozen and paraffin): 1:800-1:1500 Immunohistochemistry (PAP): 1:300-1:500 using FITC with overnight incubation at 4ºC
Optimal dilutions to be dtermined by the researcher.
Positive Control:Formalin-fixed paraffin and frozen sections of human or rat pancreas.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Dilute further with 0.1M PBS, 1% BSA, 0.09% sodium azide.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ul
Host:rabbit
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder. Reconstitute with 50-100ul sterile ddH20. Dilute further with 0.1M PBS, 1% BSA, 0.09% sodium azide.
Specificity:Recognizes Amylin. Species Crossreactivity: human, rat, feline, porcine and guinea pig.