VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm.
Epitope identified aa726-733 (C-terminus; common to all 3 VP proteins) as the specific binding region.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence/Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:200-1:500Immunoprecipitation: 1:10Immunofluorescence (IC): 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS containing stabilizing protein and 0.09% sodium azide. Use PBS for further dilutions.
Specificity：Recognizes VP1, VP2 and VP3 of Adeno-associated Virus 2. Also recognizes serotypes AAV1 through AAV-12 (see reference 1 by Sonntag, et al.).
Isotype：IgG
Calc Applications Abbrev：E IC IF IP WB
Immunogen：Recombinant adeno-associated virus (AAV-2) capsid proteins, expressed in baculovirus.
