For enhancement of cortex staining, preincubate fixed sections with Triton X-100 (in PBS) for 1-5 minutes prior to first antibody incubation step.
Incubate for 1 hour at RT
Western Blot (ECL): 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:guinea pig
Source Antibody:human
Grade:Serum
Purity:Serum
Form:Supplied as a liquid, 0.09% sodium azide
Specificity:Recognizes human hair keratin K37 expressed starting from the middle cortex of vellous hairs. Negative with cells in the anagen hair follicle. Species Crossreactivity: chimpanzee
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to CGP VTG GSP SGH GAS MGR of human acidic hair (trichocytic) keratin K37 conjugated to KLH (Formally designated keratin hHa7).