For enhancement of cortex preincubate fixed sections with Triton X-100 (in PBS) for 1-5 minutes prior to first antibody incubation step.
Incubate for 1 hour at RT
Western Blot (ECL): 1:2000 Incubate for 1 hour at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：guinea pig
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.5% BSA, 0.09% sodium azide.
Specificity：Recognizes human hair keratin K36 expressed starting from the middle region of cortex.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to APQ VGT QIR TIT EEI C of human acidic hair (trichocytic) keratin K36 conjugated to KLH. (Formally designated keratin hHa6)
