The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins.
Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2.
Applications:Suitable for use in Western Blot and Immunohistochemistry
Other applications have not been tested.
Recommended Dilutions:Western Blot (ECL): 1:2000Immunohistochemistry (Frozen): 1:200.
For enhancement of cortex staining, we recommend preincubating fixed sections with 0.1% Triton X-100 in PBS for 1-5 minutes prior to first antibody incubation step.
Incubate for 1 hour at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：guinea pig
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.09% sodium azide.
Specificity：Recognizes human hair keratin K35 expressed starting from the upper part of hair matrix, lower cortex and hair cuticle. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human acidic hair (trichocytic) keratin K35, conjugated to KLH. (Formally designated keratin hHa5;YSS SPC KLP SLS PVA RS)
