For enhancement of cortex preincubate fixed sections with Triton X-100 (in PBS) for 1-5 minutes prior to first antibody incubation step.
Incubate for 1 hour at RT
Western Blot (ECL): 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：guinea pig
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.09% sodium azide
Specificity：Recognizes human hair keratin K33 (hHa3) expressed starting from the middle hair cortex. Because of high sequence homology both keratin isoforms K33a (hHa3-I) and K33b (hHa3-II) are detected. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to EKP IGS CVT NPC GPR SR of human hair (trichocytic) keratin K33 conjugated to KLH (Formally designated keratin hHa3).
