XAF-1 binds to XIAP, an inhibitor of caspases -3, -7, and -9, and triggers its relocation from the cytosol to the nucleus (1,2).
Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death (1).
XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cencer cell lines (3).
In contrast, XIAP levels have been shown to be high in a majority of cell lines.
Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines (2,3).
Both IFN-a2 and IFN-b can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs (4).
Applications:Can be used for the detection of XAF-1 by Western blot at 0.5-2ug/ml.
Human spleen cell lysate can be used as positive control and a 32kD band can be detected.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes human XAF-1. Species Crossreactivity: Mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1 (Genbank accession CAA68030)
