A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome) (1-2).
Coronaviruses are a major cause of upper respiratory diseases in humans (3).
The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length.
SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139kD protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells.
This binding can be blocked by a soluble form of ACE2 (4).
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: Detects 10ng of free peptide at 1ug/ml
Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes SARS Spike protein (IN1).
Isotype：IgG
Calc Applications Abbrev：E
Immunogen：Synthetic peptide corresponding to amino acids 426-441 of the SARS Spike glycoprotein.
