A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome) (1-2).
Coronaviruses are a major cause of upper respiratory diseases in humans (3).
The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length.
The M protein (Membrane protein, Matrix protein) is one of the major structural viral proteins.
It is an integral membrane protein involved in the budding of the viral particles and interacts with S (Spike) protein and the nucleocapsid protein (4-5).
Applications:Can be used for the detection of SARS M protein in ELISA.
It will detect 10ng of free peptide at 1mg/ml.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity:Recognizes SARS M protein.
Isotype:IgG
Calc Applications Abbrev:E
Immunogen:Raised against a synthetic peptide corresponding to amino acids at the carboxy-terminus of the SARS M protein (Genbank accession no. P59596)