A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome) (1-2).
Coronaviruses are a major cause of upper respiratory diseases in humans (3).
The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length.
The SARS E protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers (4), which may explain its role in viral budding and virion envelope morphogenesis (5).
Applications:Can be used for the detection of SARS E protein in ELISA.
It will detect 10ng of free peptide at 1mg/ml.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity:Recognizes SARS E protein.
Isotype:IgG
Calc Applications Abbrev:E IF IHC
Immunogen:Synthetic peptide corresponding to 10aa near the amino-terminus of the SARS E protein. Epitope: within the first 50aa of SARS-CoV-2 envelope