A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome) (1-2).
Coronaviruses are a major cause of upper respiratory diseases in humans (3).
The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length.
The SARS E protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers (4), which may explain its role in viral budding and virion envelope morphogenesis (5).
Applications:Can be used for the detection of SARS E protein in ELISA.
It will detect 10 ng of free peptide at 1 mg/ml.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes SARS E protein.
Isotype：IgG
Calc Applications Abbrev：E
Immunogen：Raised against a synthetic peptide corresponding to amino acids at the carboxy-terminus of the SARS E protein (Genbank accession no. P59637)
