The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF) (1 for review).
The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR (2).
Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac).
Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization.
It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed (5).
Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells (3), NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage (4).
Applications:Can be used for detection of NADE by Western blot at 0.5 to 2ug/ml.
Despite its predicted molecular weight, NADE migrates at ~23kD in SDS-PAGE.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes human NADE. Species Crossreactivity: Mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to 14 amino acids near the middle of human NADE (GenBank accession no. NP_996798)
