84-1061-98　Livin (ML-IAP) 100ug　L3180-02

特徴

• Apoptosis, or programmed cell death, is related to many diseases, such as cancer.
• Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins.
• IAP proteins form a conserved gene family that binds to and inhibits cell death proteases.
• A novel member in the IAP protein family was recently identified and designated Livin and KIAP for kidney IAP (1,2).
• Livin/XIAP contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin-a and Livin-b (1,3).
• Transfection of Livin in cells resulted in protection from apoptosis induced by FADD, BAX, RIP, RIP3 and DR6 (1).
• Livin has direct interaction with several caspases including caspase-3, -7, and Ð9.
• Livin inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP.
• The two isoforms of Livin appear to have different functions and tissue distributions.
• Applications:Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5ug/ml.
• A band at 33kD (predicted MW) can be detected.
• A lower but much weaker ban at 30kD was detected in Raji cell lysate, which may represent the short form of Livin
• Immunohistochemistry: 5ug/ml
• Immunofluorescence: 20ug/ml
• Optimal dilutions to be determined by the researcher.
• Positive Control:Raji cell lysate; human small intestine tissue lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.