The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands.
Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis.
At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place.
Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia.
Applications:Suitable for use in Western Blot, Immunohistochemistryand Immunocytochemistry.
Other application not tested.
Recommended Dilutions:Western Blot: 1:250 (ECL)Immunohistochemistry: 1:100Immunocytochemistry: 1:100Optimal dilutions to be determined by the researcher.
Positive Control:LDLR transfected 293 cell lysate
Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken-HRP Rb x Ch I1906-12IgY, Chicken-FITC Rb x Ch I1906-14IgY, Chicken-AP Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, before the addition of glycerol to 40%.
Specificity：Recognizes human LDLR. Species Crossreactivity: Mouse and rat.
Isotype：IgY
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa29-205 of LDLRProtein Accession Number: NP_000518.1Protein GI Number: 4504975
