Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA (1).
Novel influenza virus strains caused by genetic drift and viral recombination to which humans have little or no immunity, emerge periodically, resulting in devastating pandemics.
Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found (2).
These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes.
During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong (3).
There was some evidence of human to human spread of this virus, but it is thought that the transmission efficiency was fairly low (4).
HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues.
Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability (5).
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilution:ELISA: 1ug/ml will detect 10ng of free peptide
Optimal dilutions to be determined by the researcher.
Control Peptide:I7649-41A: Influenza A, Avian, H5N1, Hemagglutinin (HA) (NT) Control Peptide (Bird Flu)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, before the addition of glycerol to 40%.
Specificity：Recognizes Hemagglutinin from Avain Influenza A (H5N1) virus.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Synthetic peptide corresponding to 15aa at the N-terminus of the Hemagglutinin protein (Genbank accession no. AAT76166). Efforts were made to use relatively conserved regions of the viral sequence as the antigen.
