ILPIP (ILP-interacting protein) has recently been shown to interact with XIAP, a member of the IAP (Inhibitor of Apoptosis) protein family (1).
Together, these two proteins synergistically activate the TAK1/JNK1 signal transduction pathway, which acts to protect against the interleukin-1b converting enzyme of Fas-induced apotosis (1,2).
This protein was independently isolated and characterized as polyploidy-associated protein kinase (PAPK), a member of the Ste20/germinal center kinase family that modulates cytoskeletal organization and cell survival (3), suggesting that its protective role may be a function of its kinase activity.
Although two mRNA sequences have been isolated, only the shorter isoform has been observed.
Applications:Can be used for the detection of ILPIP by Western Blot at 1 to 2ug/ml.
Human brain cell lysate can be used as positive control and a 34kD band can be detected.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes human ILPIP. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids near the center of human ILPIP (Genbank accession NP_061041)
