This protein may play a role in maintaining stem cell self-renewal.
Depletion or overexpression of nucleostemin reduces cell proliferation in CNS stem cells and transformed cells.
Mutation analysis indicated that excessive nucleostemin, particularly mutants lacking the GTP-regulatory domain, prevents cells from entering mitosis and causes apoptosis in a p53-dependent manner.
The N-terminal basic domain mediates the interaction with p53 and is required for the cell death caused by overexpression.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutiion:Western blot: 1:250
Immunohistochemistry: 1:100.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：chicken
Source Antibody：human
Grade：Purified
Purity：Purified.
Form：Supplied as a liquid in PBS, pH 7.4, before the addition of glycerol to 40%.
Specificity：Recognizes human E2IG3. Species Crossreactivity: mouse and rat.
