Three distinct signaling pathways lead to programmed cell death (apoptosis).
The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved.
The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2).
Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion.
Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis.
Caspase-12 is colocalized to the ER with several proteins that are involved in Alzheimer's disease including g-secretase presenilin and b-amyloid precursor protein (APP).
Caspase-12 mediates cytotoxicity induced by amyloid-b.
Caspase-12 is ubiquitously expressed in mouse tissues (1).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry: 2ug/ml; Stains human heart tissue
Optimal dilutions to be determined by the researcher.
Positive Control:Human heart cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes mouse Caspase 12. Species Crossreactivity: Human and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids within the large cleavage product of murine caspase-12 (Genbank accession CAA73532)
