Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death.
Bcl-2 homology 3 (BH3) domain is a potent death domain (reviewed in 1 and 2).
BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family.
A novel BH3 domain containing protein was recently identified and designated Bcl-G (3).
The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis.
The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol.
Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS.
Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein (3).
Applications:Can be used for detection of Bcl-G by Western blot at 2.5 to 5ug/ml.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human Bcl-G. Although this antibody should react with both isoforms, only the Bcl-GS protein has been observed. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to 15 amino acids near the C-terminus of human Bcl-G (GenBank accession no. NM_030766)
