Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase.
A substantial quantity of tryptase is estimated to be found in mast cells of skin and lung and suggestes this enzyme plays a major role in mast cell mediated events.
In vitro studies indicate tryptase can cleave C3 to form C3a anaphylatoxin, inactivate fibrinogen as a coaguable substrate for thrombin and activate latent collagenase.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1ug/mlImmunoprecipitation: 25ug/ml conditioned cell culture medium spiked with reconbinant mouse Tryptase, beta1Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Tryptase, beta1 in direct ELISAs and Western Blots. Shows ~10% crossreactivity with recombinant human TPSB2, 5% crossreactivity with recombinant human TPS1 and <1% crossreactivity with recombinant human TPSG1 and recombinant mouse MCPT1.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ile29-Phe273 from mouse Tryptase beta1 expressed in NS0 cells
