84-1061-35　Fractin 100ul　F6070-20

特徴

• During apoptosis, beta actin is cleaved by activated caspase 3 between Asp244 and Gly245.
• This cleavage results in the release of an approximately 130 amino acid (15kD) fragment from the C-terminus of beta actin and a 32kD (244 amino acid) N-terminal fragment of actin, known as Fractin.
• The C-terminus of the Fractin fragment represents an apoptosis-specific neoepitope that is not antigenic in native full-length beta actin and therefore presents a novel and specific marker for distinguishing apoptotic from necrotic cells.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000-1:4000 for incubation overnight at 4°C.
• Recommended antibody dilution buffer: TBS with 3% BSA and 0.05% Tween-20, pH 7.4
• Immunohistochemistry/Immunocytochemistry: 1:400-1:1000.
• Incubate 1 hr at 37°C or 4°C overnight.
• Paraffin-embedded tissues may require antigen retrieval methods
• Optimal dilutions to be determined by the researcher.
• Positive Control:Staurosporin treated neuronal cultures
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.