SFRP3, also known as FRZB, Fritz, Frzb1 and FRP-3, is expressed in the developing bone and cartilage as well as several adult tissues.
It functions as an antagonist of Wntsignaling.
Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.2ug/ml; The detection limit for recombinant mouse SFRP3 is ~5ng/lane under non-reducing and reducing conditions.
In this format, shows ~60% crossreactivity with recombinant human SFRP3
Immunohistochemistry: 2-5ug/ml; Detects SFRP3 in cells and tissues
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile TBS, 0.1% BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS, pH 7.3, 0.1% BSA.
Specificity：Recognizes mouse SFRP3.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ala33-Ser323 from mouse SFRP3, expressed in NS0 cells.
