Secreted Frizzled Related Protein 3 (sFRP-3) was originally identified in bovine cartilage for its chondrogenic ability.
Human, mouse, chick and Xenopus clones have also been isolated.
SFRP-3 is often referred to as FRZB, other names include Fritz, Frzb1, and FRP-3.
At the amino acid sequence level, sFRP-3 is highly conserved.
The human protein shares 77% identity with Xenopus, 92% with mouse, and 94% with bovine proteins.
Human sFRP-3 is strongly expressed in the developing appendicular skeleton, and cartilage of craniofacial bones.
As determined by Northern blot of adult tissues, it is strongly detected in heart and placenta, as well as the brain, skeletal muscle, kidney and pancreas.
The N-terminal portion of the human protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals.
The similarity of sFRP-3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them.
SFRP-3 was subsequently shown to be a soluble antagonist of Wnt signals.
It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts.
Ectopic expression of sFRP-3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.2ug/ml.
Used with the appropriate secondary reagents to detect human and mouse sFRP-3.
The detection limit for rhsFRP-3 and rmsFRP-3 is ~5ng/lane and 50ng/lane under non-reducing and reducing conditions, respectively
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile TBS, pH 7.3, 0.1% BSA.
Aliquot to avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS, pH 7.3, 0.1% BSA.
Specificity：Recognizes human sFRP-3. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human secreted FRP-3, expressed in NS0 cells.
