Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes.
Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain and a variable-length C-terminal tail.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/ml.
Detection limit for recombinant mouse Frizzled-8 is ~5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, with BSA. Reconstitute with 1ml of TBS, 0.1% BSA. Labeled with Biotin.
Specificity：Recognizes mouse Frizzled-8. Less than 5% crossreactivity with recombinant mouse Frizzled-1, Frizzled-2, Frizzled-3, Frizzled-4, Frizzled-6, Frizzled-7 and Frizzled-9.
