Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes.
Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain and a variable-length C-terminal tail.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
The detection limit for recombinant mouse Frizzled-1 is ~2ng/lane under non-reducing and reducing conditions.
In this format, this antibody shows approximately 5% crossreactivity with recombinant mouse Frizzled-3, -4, -7, -8 and less than 1% crossreactivity with recombinant mouse Frizzled-6
Immunohistochemistry: 15ug/ml detects Frizzled-1 in cells and tissues
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile Tris-buffered saline, 0.1% BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.3, 0.1% BSA. Labeled with Biotin. Reconstitute with 1ml sterile Tris-buffered saline pH 7.3, 0.1% BSA.
Specificity：Recognizes mouse Frizzled-1.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to the extracellular Cysteine-rich Domain (CRD) of mouse Frizzled-1, expressed in NSO cells.
