Applications: Direct ELISA, Western blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Direct ELISA: 0.5-1ug/ml
Western blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-6.
The detection limit for rmFrizzled-7 is approximately 1ng/lane under non-reducing and reducing conditions
Immunohistochemistry: 1.7-15ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, before the addition of 40% glycerol.
Specificity：Recognizes mouse Frizzled-7 extracellular Cysteine-Rich Domain (CRD). Approximately 15% crossreactivity with rmFrizzled-1, 5% crossreactivity with rmFrizzled-3 and rmFrizzled-6 and less than 2% crossreactivity with rmFrizzled-4.
