The signal recognition particle (SRP) is an oligomeric complex that mediates targeting and insertion of the signal sequence of exported proteins into the membrane of the endoplasmic reticulum.
SRP consists of a 7S RNA and six protein subunits.
54kD protein (SRP54) is one of these subunits.
It is a GTP-binding protein that interacts with the signal sequence when it emerges from the ribosome.
The 54kD subunit of the signal recognition particle has a two domain structure: the G-domain that binds GTP and the M-domain that binds the 7s RNA and also binds the signal sequence.
The N-terminal 300 residues of SRP54 include the GTP-binding site (G-domain) and are evolutionary related to similar domains in other proteins.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken-HRP Rb x Ch I1906-12IgY, Chicken-FITC Rb x Ch I1906-14IgY, Chicken-AP Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human Signal Recognition Particle, 54kD. Species Crossreactivity: Mouse and rat.
Isotype：IgY
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to aa300-504 from human SRP54.
