Alpha-fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life.
Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma.
However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology.
The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4.
Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin.
The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Applications:Suitable for use in ELISA and Western Blot.
May be suitable for use in Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human a1-Fetoprotein.
Isotype：IgY
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa180-420 from human a1-Fetoprotein.
