Estrogen receptor (ER) has two distinct isoforms, alpha and beta.
ER, the steroid receptor, belongs to a large superfamily of ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone.
The estrogen receptor is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription.
Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions.
The translated receptors show less variability.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human ERa. Species Crossreactivity: Mouse and rat.
Isotype：IgY
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa255-367 from human ERa.
