Complement C3 contains two chains linked by a disulfide bond.
Its activation by a C3 convertase releases the C3a anaphylatoxin from the amino end of the alpha chain and generates C3b, which associates with the Bb fragment of complement factor B to form the alternative-complement- pathway C3/C5 convertase.
C3a anaphylatoxin is a vasoactive peptide and a mediator of inflammation.
C3b, with its highly reactive thiol group, binds to the surface of foreign particles and facilitates phagocytosis.
It binds to complement C5 and renders it susceptible to proteolysis by the classical-complement-pathway C3/C5 convertase.
The activity of C3b is regulated by proteolytic cleavage involving factors H and I.
Its degradation products can also be biologically active.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1903-80: IgY, H&L, X-Adsorbed, 12nm (Gold) Pab Dk x ChI1903-80A: IgY, H&L, X-Adsorbed, 18nm (Gold) Pab Dk x ChI1903-80B: IgY, H&L, X-Adsorbed, 6nm (Gold) Pab Dk x ChI1906: IgY Pab Rb x ChI1906-01: IgY, Fc (HRP) Pab Gt x ChI1906-10: IgY (HRP) Pab Rb x ChI1906-12: IgY (FITC) Pab Rb x ChI1906-14: IgY (AP) Pab Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human Complement C3.
Isotype：IgY
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1-1663 from human Complement C3.
