Human serum albumin is a single chain polypeptide which composes about 60% of the plasma proteins.
In normal serum approximately 10% of the albumin is modified by non-enzymatic glycosylation which occurs primarily on lysine residue number 525.
The amount of glycosylated albumin in serum is markedly elevated in diabetes and thus its determination may help in the monitoring of the disease.
Studies also show that glycosylation of the protein alters its properties and this modification causes it to be ingested by endothelial cells in preference to unmodified albumin.
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs.
Its main function is the regulation of the colloidal osmotic pressure of blood.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: IndirectWestern Blot: 1:500Optimal dilutions to be determined by the researcher.